Technology for Life
Rat Ultra Sensitive Insulin Elisa Kit
(Low and High Range Assay)
 
Low and High Range Rat Insulin Assay Using the Same Kit
Sample Size
5µl
 
Samples:
Serum, plasma, or fluid
 
Tests:
96 wells (8 wells x 12 modules)
 
Reagents:
In liquid form (except standard)
 
Assay Range:
Low Range Assay: 0.1 - 6.4 ng/ml
Wide Range Assay: 0.1 - 12.8 ng/ml*
High Range Assay: 1 - 64 ng/ml
* Intended for screening purpose
 
Assay Time:
Same day procedure
 
Precision:
Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Recovery:
When rat insulin was spiked to rat serum, the recovery of rat insulin was 100% ± 10%
Specificity:
Substance         
Rat Insulin         
Mouse Insulin       
Hamster Insulin         
Rat C-peptide         
Rat pancreatic polypeptide         
Porcine glucagon         
Human Insulin like growth factor-I
Reactivity
100%
100%*
Detected*
Not detected
Not detected
Not detected
Not detected
Not detected
Typical Standard
Curve:
Highlighted
Features:
  • Small Sample Volume: Only 5µl
  • High Sensitivity: 100 pg/ml using 5µl sample & 5 pg/ml using 100µl sample
  • Low and High Insulin Assay: Both using the same kit
  • Precision: Intra-assay and inter-assay precision CV ≤ 10.0%
  • Protocol: Same protocol for serum, plasma, or fluid assay
  • Effect of Hemolysis: Almost no effect of hemolysis tested up to 20mg/ml.
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